Prevalence of Trichomonas vaginalis Infection among Women of Reproductive Age in Anambra State, South Eastern, Nigeria

J. C. Ozougwu *

Department of Biological Sciences, Rhema University Nigeria, Aba, Abia State, Nigeria.

C. A. Imakwu

Department of Parasitology and Entomology, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

I. Nwachukwu

Department of Microbiology, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria.

O. A. Okeke

Department of Zoology, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.

*Author to whom correspondence should be addressed.


Aim: This study was undertaken to determine the prevalence of Trichomonas vaginalis infection among women of reproductive age in some selected hospitals in Awka, Anambra State, Nigeria.

Methods: This study, conducted from October to December 2021, involved 200 women aged 20 years and above as participants. Prior to specimen collection, informed consent was obtained from each woman by qualified medical personnel. High vaginal swab sticks, labeled and sterile, were used to meticulously and aseptically collect specimens from the high vaginal area. A few drops of normal saline were added to each swab stick, and within 30 minutes of collection, a wet mount examination was performed to ensure optimal results. For the wet preparation of vaginal discharge, a drop from the sample was applied to a clean glass slide with a cover slip, avoiding the trapping of air bubbles. The wet smear was then examined under a microscope using a low-power objective (x10) followed by a high-power objective (x40) to detect motile T. vaginalis. The vaginal secretion was characterized by the presence of epithelial cells, white blood cells, and red blood cells. The T. vaginalis trophozoite was identified based on its oval shape, flagellation, and distinct jerky movement.

Results: The study area had an overall prevalence rate of 11.0% for T. vaginalis infection. Among the selected hospitals, COOUTH had the highest prevalence at 13.24%, while Rock Foundation Hospital had the lowest at 6.67%. Prevalence varied across age groups, with the highest rate of 16.42% found in the 30-39 years age group, and the lowest rate of 5.0% in the 20-29 years age group. Divorced women had the highest prevalence at 60.0%, while singles had the lowest at 4.7% among the marital groups. Among different occupations, traders had the highest prevalence at 12.24%, whereas students and civil servants had the lowest rates at 4.0% and 7.72%, respectively. T. vaginalis infection was only observed in non-pregnant women, with a prevalence rate of 13.3%.

Conclusion: T. vaginalis infection is prevalent among sexually active women of reproductive age in the study area. The findings highlight the importance of practicing good personal hygiene and being faithful to a single sexual partner. It is recommended to raise public awareness regarding the prevention and control of T. vaginalis in order to reduce its prevalence among women in the study area.

Keywords: Prevalence, Trichomonas vaginalis, infection, women, reproductive age, Anambra

How to Cite

Ozougwu , J. C., Imakwu , C. A., Nwachukwu , I., & Okeke , O. A. (2023). Prevalence of Trichomonas vaginalis Infection among Women of Reproductive Age in Anambra State, South Eastern, Nigeria. International STD Research & Reviews, 12(2), 1–7.


Krieger JN. Trichomonas vaginalis and Trichomoniasis. McGraw-Hill New York. 2010; 26:587-589.

Ogomaka IA, Nwachinemere AV, Obeagu EI. Prevalence of trichomoniasis among adults in Oru-East L.G.A, Imo State, Nigeria. Medical Public Journals. 2018;9:1-2.

Auta IK, Ibrahim B, Henry, D. Prevalence of Trichomonas vaginalis among pregnant women attending antenatal clinic in two health facilities within Kaduna Metropolis, Kaduna, Nigeria. Science World Journal. 2020;15(1):89-99.

Swygard H, Sena AC, Hobbs MM, Gohen MS. Trichomoniasis clinical manifestations diagnosis and management. Sexually Transmitted Infections. 2004;80:91-95.

World Health Organization. Global Prevalence and Incidence of selected Curable Sexually Transmitted Diseases Overview and Estimates. World Health Statistics; 2012.

Okoko FJ. Prevalence of Trichomoniasis among Women at Effurun Metropolis, Delta State, Nigeria. Crescent Journal of Biological Sciences. 2011;4(2):45-48.

Sam-wobo SO, Ajao OK, Adeleke MO, Ekpo UF. Trichomoniasis among Antenatal Attendees in a Tertiary Health Facility, Abeokuta, Nigeria. Munis Entomology and Zoology Journal. 2012;7(1):380-384.

Chinedum OK, Ifeanyi OE, Ugwu UG, Ngozi GE. Prevalence of Trichomonas vaginalis among pregnant women attending hospital in Irrua Specialist Teaching Hospital in Edo State. Nigeria Journal of Medical and Dental Sciences. 2014;13(9): 79-82.

Smith DA. Ramos N. Trichomoniasis. E-Medicine Specialties; 2010. Available:

Center for Disease Control and Prevention. Sexually Transmitted Diseases Treatment Guideline: Morbidity and Mortality Weekly Report. 2006;55:1-94.

Johnson HL, Ghanem KG, Zenilman JM. Erbelding EJ. Sexually transmitted infections and adverse pregnancy outcomes among women attending inner city public sexually transmitted diseases clinics. Sexually Transmitted Diseases. 2011;38:167-171.

Silver BJ, Guy RJ, Kaldor JM, Jamil MS, Rumbold AR. Trichomonas vaginalis as a cause of perinatal morbidity: A systematic review and meta-analysis. Sexually Transmitted Disease. 2014;41:369–376.

Kissinger P. Adamsku A. Trichomoniasis and HIV interactions: A review. Sexually Transmitted Infection. 2013;89:426–433.

Onyido AE, Umeanaeto PU, Irikannu KC, Ekwunife CA, Ezeanya LC, Nwangwu UC, Ugha CN, Obiechina IO. Prevalance of Trichomonas vaginalis among the rural women of Ekwumili Community, Anambra State, Southeastern Nigeria, Nature and Science. 2014;12(8).

Gundiri MA, Okwuosa VN. Prevalence of urinary and intestinal tracts parasites in Kwampe, Langtong, North Nigeria. Nigerian Journal of Parasitology. 2005;26: 19-22.

Obiukwu MO, Onyido AE, Duru JU, Aleke O. Trichomonas vaginalis infection in Anambra State: Demography and Behavioural Predictors. Journal of Advancement in Medical and Pharmaceutical Sciences. 2010;4(1).

Iwueze MO, Ezeanya LN, Okafor FC, Nwaogu OC, Ukibe SC. Prevalence of Trichomonas vaginalis infection among women attending hodpitals/health centers in Onitsha Community, Onitsha North Local Government Area of Anambra State. The Bioscientists. 2014; 2(1):54-64.

Workowski KA, Bolan GA. Sexually transmitted diseases treatment guidelines. Centers for Disease Control and Prevention. 2015;64(3):1-37.