Detection of Treponema pallidum (Syphilis) Antibodies among HIV-Infected Individuals and Sexually-active Attendees of Two Health Facilities in Port Harcourt, Nigeria

Main Article Content

Iheanyi O. Okonko
Tochi I. Cookey
Sofiat Adewuyi-Oseni
Amaka M. Awanye

Abstract

Aim: This study reports on the detection of Treponema pallidum (syphilis) antibodies in HIV infected patients in Port Harcourt, Nigeria. Screening for syphilis was carried out to determine the prevalence levels of these infections, as biological markers of risk, modes, and time functions of their transmission.

Study Design: Cross-sectional study.

Place and Duration of Study: University of Port Harcourt Teaching Hospital (UPTH) and O.B. Lulu Briggs Medical Centre, University of Port Harcourt, both in Port Harcourt, Nigeria, between August 2012 and July 2015.

Methods: A total of 100 HIV-infected individuals and 100 sexually-active attendees were recruited for this study. Samples of blood were collected and re-screened for the presence of HIV antibodies using the Determine HIV-1/2 (Alere), HIV ½ Stat-Pak (Chembio), and HIV-1/2/P24/O ELISA kit (Dia.Pro). The same set of samples were screened for Treponema pallidum specific antibodies using the syphilis Ultra Rapid Test Strip (ACON(R), USA) and syphilis rapid strips (Global, USA) following the respective manufacturer's instructions.

Results: Among the 200 samples, serological reactivity was detected for syphilis in 3(1.5%). The incidence of syphilis was higher in males (2.0%) than in females (1.0%). Age, sex, and locality did not significantly (P>0.05) influence the rate of syphilis.

Conclusion: This study further confirms the presence of syphilis among the population studied. Routine screening of Syphilis among patients is therefore advocated.

Keywords:
Treponema pallidum, syphilis, HIV, epidemiology, Nigeria

Article Details

How to Cite
Okonko, I. O., Cookey, T. I., Adewuyi-Oseni, S., & Awanye, A. M. (2020). Detection of Treponema pallidum (Syphilis) Antibodies among HIV-Infected Individuals and Sexually-active Attendees of Two Health Facilities in Port Harcourt, Nigeria. International STD Research & Reviews, 9(2), 17-23. https://doi.org/10.9734/ISRR/2020/v9i230110
Section
Original Research Article

References

Department of Health. 2000. HIV/ AIDS/STD strategic plan for South Africa: 2000-2005. Pretoria, South Africa: Department of Health; 2000.

Golden MR, Hogben M, Handsfield HH, St Lawrence JS, Potterat JJ, Holmes KK. Partner notification for HIV and STD in the United States: low coverage for gonorrhoea, chlamydial infection, and HIV. Sexually Transmitted Diseases. 2003;30: 490-496.

World Health Organization, Geneva; 2001.
Available:https://www.who.int/publishers/en.html
Accessed July 2020.

Azuonwu G, Timothy TE. Overview of Prevalence of Syphilis in a Health Facility in Rivers State. International STD Research & Reviews. 2020;9(2):1-7.

Okonko IO, Okerentugba PO, Adejuwon AO. Prevalence of sexually transmitted infections (STDs) among attendees of Lead City University Medical Centre in Ibadan, Southwestern Nigeria. Archives of Applied Science Research. 2012; 4(2): 480-487.

World Health Organization. WHO Technical Report Series No. 810, Geneva; 2006.

de Souza RL, Madeira LDD, Pereira MVS, da Silva RM, de Luna Sales JB, Azevedo VN, Feitosa RNM, Monteiro JC, Ishak MDG, Ishak R, Ribeiro ALR, Oliveira-Filho AB, Machado LFA. Prevalence of syphilis in female sex workers in three countryside cities of the state of Pará, Brazilian Amazon. Bio-Medical Center of Infectious Diseases. 2020; 20:129.

Right Diagnosis. Prevalence and Incidence of Syphilis; 2015.
Available:https://www.rightdiagnosis.com/s/syphilis/prevalence.htm
Accessed August 8, 2020.

Opone CA, Abasiattai AM, Utuk MN, Bassey EA. The prevalence of syphilis in pregnant women in Akwa Ibom State, Southern Nigeria. Tropical Journal of Obstetrics and Gynaecology. 2020; 36(2): 224-231.

Macfarlane SB. Conducting a Descriptive Survey: 2. Choosing a Sampling Strategy. Tropical Doctors. 1997; 27(1):14-21.

Naing L, Winn T, Rusli BN. Practical issues in calculating the sample size for prevalence. Studies Archives of Orofacial Sciences. 2006; 1:9–14.

World Health Organization; 1991. Management of Patients with Sexually Transmitted Diseases. WHO Technical Report Series No. 810. Geneva: World Health Organization.

Cheesbrough M. Medical Laboratory Manual for Tropical Countries ELBS. 2006; 7:312-315.

Noris SJ. Treponema and other human hosts- associated Spirochetes. In: Manual of Clinical Microbiology, 8th edition Murray P. R et al., (Editors). Amsterdam Society of Medicine Press; 2003.

Mutagoma M, Remera E, Sebuhoro D, Kanters S, Riedel DJ, Nsanzimana S. The Prevalence of Syphilis Infection and Its Associated Factors in the General Population of Rwanda: A National Household-Based Survey. Journal of Sexually Transmitted Diseases. 2016; 2016, Article ID 4980417.

Chad HH, Schneider JA, Dandona R. Population and dyadic-based seroincidence of herpes simplex virus-2 and syphilis in southern India. Sexually Transmitted Infections. 2015; 91(5):375–382.

Otieno-Nyunya B, Bennett E, Bunnell R, et al. Epidemiology of syphilis in Kenya: results from a nationally representative serological survey. Sexually Transmitted Infections. 2011;87(6):521–525.

Ojo AD, Oyetunji AI. Sero-prevalence of syphilis among pregnant women in Osogbo in Southwestern Nigeria. International Journal of Environmental Science and Technology. 2007; 6:61-65.

Buseri FI, Seiyaboh E, Jeremiah ZA. Surveying Infections among pregnant women in the Niger Delta, Nigeria. Journal of Global Infectious Diseases. 2010; 2:203-211.

Adjei AA, Kudzi W, Armah H, et al. Prevalence of antibodies to syphilis among blood donors in Accra, Ghana. Japanese Infectious Diseases. 2003; 56:165– 167.

Scherbaum N, Baune BT, Mikolajczyk R. et al. Prevalence and risk factors of syphilis infection among drug addicts. Bio-Medical Center of Infectious Diseases. 2005, 5:33.

Ibadin KO, Enabulele OI, Eghafona NO, Osemwenkha AP. Serodynamics of Treponema pallidum in pregnant women. Benin Journal of Postgraduate Medicine. 2009; 1:9-12.

Olokoba AB, Salawu FK, Danduram A, Desalu OO, Midala JK, Badung LH, Lateefat O, Abdulkarim A. Syphilis in pregnant women: Is it still necessary to screen? European Journal of Science Research. 2009; 29:315-319.

Ejele OA, Erhabor O, Nwauche CA. Trends in the prevalence of some transfusion-transmissible infections among blood donors in Port Harcourt, Nigeria. Haematology. 2005; 8:273– 277.

Aboyeji AP, Nwabuisi C. Prevalence of sexually transmitted diseases among pregnant women in Ilorin, Nigeria. Journal of Obstetrics and Gynaecology. 2003; 23: 637-639.

World Health Organization, Global Incidence and Prevalence of Selected Curable Sexually Transmitted Infections, World Health Organization; 2012.

Hussain T, Kulshreshtha KK, Sinha S, Yadav VS, Katoch VM. HIV, HBV, HCV, and Syphilis c-infections among patients attending the STD and clinics of district hospitals in Northern India. International Journal of Infectious Diseases. 2006; 10:358-363.